Dataset Information

In vitro reconstruction and analysis of evolutionary variation of the tomato acylsucrose metabolic network.

ABSTRACT: Plant glandular secreting trichomes are epidermal protuberances that produce structurally diverse specialized metabolites, including medically important compounds. Trichomes of many plants in the nightshade family (Solanaceae) produce O-acylsugars, and in cultivated and wild tomatoes these are mixtures of aliphatic esters of sucrose and glucose of varying structures and quantities documented to contribute to insect defense. We characterized the first two enzymes of acylsucrose biosynthesis in the cultivated tomato Solanum lycopersicum. These are type I/IV trichome-expressed BAHD acyltransferases encoded by Solyc12g006330--or S. lycopersicum acylsucrose acyltransferase 1 (Sl-ASAT1)--and Solyc04g012020 (Sl-ASAT2). These enzymes were used--in concert with two previously identified BAHD acyltransferases--to reconstruct the entire cultivated tomato acylsucrose biosynthetic pathway in vitro using sucrose and acyl-CoA substrates. Comparative genomics and biochemical analysis of ASAT enzymes were combined with in vitro mutagenesis to identify amino acids that influence CoA ester substrate specificity and contribute to differences in types of acylsucroses that accumulate in cultivated and wild tomato species. This work demonstrates the feasibility of the metabolic engineering of these insecticidal metabolites in plants and microbes.

SUBMITTER: Fan P

PROVIDER: S-EPMC4720351 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature

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In vitro reconstruction and analysis of evolutionary variation of the tomato acylsucrose metabolic network.

Fan Pengxiang P Miller Abigail M AM Schilmiller Anthony L AL Liu Xiaoxiao X Ofner Itai I Jones A Daniel AD Zamir Dani D Last Robert L RL

Proceedings of the National Academy of Sciences of the United States of America 20151229 2

Plant glandular secreting trichomes are epidermal protuberances that produce structurally diverse specialized metabolites, including medically important compounds. Trichomes of many plants in the nightshade family (Solanaceae) produce O-acylsugars, and in cultivated and wild tomatoes these are mixtures of aliphatic esters of sucrose and glucose of varying structures and quantities documented to contribute to insect defense. We characterized the first two enzymes of acylsucrose biosynthesis in th ...[more]

PMID: 26715757

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Dataset Information

In vitro reconstruction and analysis of evolutionary variation of the tomato acylsucrose metabolic network.

Publications

In vitro reconstruction and analysis of evolutionary variation of the tomato acylsucrose metabolic network.

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