Label-free proteomic analysis of the hydrophobic membrane protein complement in articular chondrocytes: a technique for identification of membrane biomarkers.

ABSTRACT:

Context

There is insufficient knowledge about the chondrocyte membranome and its molecular composition.

Objective

To develop a Triton X-114 based separation technique using nanoLC-MS/MS combined with shotgun proteomics to identify chondrocyte membrane proteins.

Materials and methods

Articular chondrocytes from equine metacarpophalangeal joints were separated into hydrophobic and hydrophilic fractions; trypsin-digested proteins were analysed by nanoLC-MS/MS.

Results

A total of 315 proteins were identified. The phase extraction method yielded a high proportion of membrane proteins (56%) including CD276, S100-A6 and three VDAC isoforms.

Discussion

Defining the chondrocyte membranome is likely to reveal new biomarker targets for conventional and biological d

SUBMITTER: Matta C 

PROVIDER: S-EPMC4819840 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature