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Mapping Local Cytosolic Enzymatic Activity in Human Esophageal Mucosa with Porous Silicon Nanoneedles.

ABSTRACT: Porous silicon nanoneedles can map Cathepsin B activity across normal and tumor human esophageal mucosa. Assembling a peptide-based Cathepsin B cleavable sensor over a large array of nano-needles allows the discrimination of cancer cells from healthy ones in mixed culture. The same sensor applied to tissue can map Cathepsin B activity with high resolution across the tumor margin area of esophageal adenocarcinoma.

SUBMITTER: Chiappini C

PROVIDER: S-EPMC4858817 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature

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Mapping Local Cytosolic Enzymatic Activity in Human Esophageal Mucosa with Porous Silicon Nanoneedles.

Chiappini Ciro C Campagnolo Paola P Almeida Carina S CS Abbassi-Ghadi Nima N Chow Lesley W LW Hanna George B GB Stevens Molly M MM

Advanced materials (Deerfield Beach, Fla.) 20150721 35

Porous silicon nanoneedles can map Cathepsin B activity across normal and tumor human esophageal mucosa. Assembling a peptide-based Cathepsin B cleavable sensor over a large array of nano-needles allows the discrimination of cancer cells from healthy ones in mixed culture. The same sensor applied to tissue can map Cathepsin B activity with high resolution across the tumor margin area of esophageal adenocarcinoma. ...[more]

PMID: 26197973

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