Effects of hemoglobin C, D, E and S traits on measurements of hemoglobin A1c by twelve methods.

ABSTRACT:

Background

Hemoglobin C, D Punjab, E or S trait can interfere with hemoglobin A1c (HbA1c) results. We assessed whether they affect results obtained with 12 current assay methods.

Methods

Hemoglobin AA (HbAA), HbAC, HbAD Punjab, HbAE and HbAS samples were analyzed on one enzymatic, nine ion-exchange HPLC and two Capillary Electrophoresis methods. Trinity ultra(2) boronate affinity HPLC was the comparative method. An overall test of coincidence of least-squared linear regression lines was performed to determine if HbA1c results were statistically significantly different from those of HbAA samples. Clinically significant interference was defined as >7% difference from HbAA at 6 or 9% HbA1c compared to ultra(2) using Deming regression.

Results

All methods showed statistic

SUBMITTER: Rohlfing C 

PROVIDER: S-EPMC4966613 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature