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MiR-17 as a diagnostic biomarker regulates cell proliferation in breast cancer.

ABSTRACT:

Background

MicroRNAs (miRNAs) have been shown to be involved in the initiation and progression of cancers in the literature. In this study, we aimed to evaluate the clinicopathological role of miR-17 in breast cancer.

Materials and methods

The expression of miR-17 was measured in 132 breast cancer tissues and paired adjacent normal tissues by using real-time quantitative polymerase chain reaction. The association between miR-17 expression levels and clinicopathological parameters was also analyzed. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry assays were used to investigate the role of miR-17 in the regulation of breast cancer cells.

Results

The expression of miR-17 was remarkably increased in breast cancer tissues and cell lines. Clinical association analysis revealed that a high expression of miR-17 was prominently associated with poor survival time in breast cancer. Overexpression of miR-17 promoted cell proliferation and induced tumor growth.

Conclusion

Our findings clarified that the upregulation of miR-17 played a vital role in breast cancer progression and suggested that miR-17 could be used as a prognostic biomarker for breast cancer.

SUBMITTER: Yang F

PROVIDER: S-EPMC5293507 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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miR-17 as a diagnostic biomarker regulates cell proliferation in breast cancer.

Yang Fangliang F Li Yuan Y Xu Lingyun L Zhu Yulan Y Gao Haiyan H Zhen Lin L Fang Lin L

OncoTargets and therapy 20170127

<h4>Background</h4>MicroRNAs (miRNAs) have been shown to be involved in the initiation and progression of cancers in the literature. In this study, we aimed to evaluate the clinicopathological role of miR-17 in breast cancer.<h4>Materials and methods</h4>The expression of miR-17 was measured in 132 breast cancer tissues and paired adjacent normal tissues by using real-time quantitative polymerase chain reaction. The association between miR-17 expression levels and clinicopathological parameters ...[more]

PMID: 28203087

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