Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling.
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ABSTRACT:
SUBMITTER: Tredwell GD
PROVIDER: S-EPMC5329079 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling.
Tredwell Gregory D GD Aw Rochelle R Edwards-Jones Bryn B Leak David J DJ Bundy Jacob G JG
Journal of industrial microbiology & biotechnology 20170203 3
Heterologous protein production in the yeast Pichia pastoris can be limited by biological responses to high expression levels; the unfolded protein response (UPR) is a key determinant of the success of protein production in this organism. Here, we used untargeted NMR metabolic profiling (metabolomics) of a number of different recombinant strains, carried out in a miniaturized format suitable for screening-level experiments. We identified a number of metabolites (from both cell extracts and super ...[more]
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