Cofactor engineering to regulate NAD⁺/NADH ratio with its application to phytosterols biotransformation.

ABSTRACT:

Background

Cofactor engineering is involved in the modification of enzymes related to nicotinamide adenine dinucleotides (NADH and NAD⁺) metabolism, which results in a significantly altered spectrum of metabolic products. Cofactor engineering plays an important role in metabolic engineering but is rarely reported in the sterols biotransformation process owing to its use of multi-catabolic enzymes, which promote multiple consecutive reactions. Androst-4-ene-3, 17-dione (AD) and androst-1, 4-diene-3, 17-dione (ADD) are important steroid medicine intermediates that are obtained via the nucleus oxidation and the side chain degradation of phytosterols by Mycobacterium. Given that the biotransformation from phytosterols to AD (D) is supposed to be a NAD⁺-dependent p

SUBMITTER: Su L 

PROVIDER: S-EPMC5663084 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature