Glycogen metabolism in mink uterine epithelial cells and its regulation by estradiol, progesterone and insulin.

ABSTRACT: Glycogen content in mink uterine glandular and luminal epithelia (GE and LE) is maximal during estrus and is depleted before implantation while embryos are in diapause. Uterine glycogen synthesis in vivo is stimulated by estradiol (E₂) while its mobilization is induced by progesterone (P₄). Nevertheless, treatment of an immortalized mink uterine epithelial cell line (GMMe) with E₂ did not affect glycogen production. Interestingly, insulin alone significantly increased synthesis of the nutrient and glycogen content in response to insulin + E₂ was greater than for insulin alone. Our objectives were to determine: 1) If insulin receptor protein (INSR) is expressed by mink uterine GE and LE in vivo and if the amount differs between estrus, diapause an

SUBMITTER: Hodonu A 

PROVIDER: S-EPMC6701937 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature