Nonequilibrium Biophysical Processes Influence the Large-Scale Architecture of the Cell Nucleus.

ABSTRACT: Model approaches to nuclear architecture have traditionally ignored the biophysical consequences of ATP-fueled active processes acting on chromatin. However, transcription-coupled activity is a source of stochastic forces that are substantially larger than the Brownian forces present at physiological temperatures. Here, we describe an approach to large-scale nuclear architecture in metazoans that incorporates cell-type-specific active processes. The model predicts the statistics of positional distributions, shapes, and overlaps of each chromosome. Simulations of the model reproduce common organizing principles underlying large-scale nuclear architecture across human cell nuclei in interphase. These include the differential positioning of euchromatin and heterochromatin, the territorial org

SUBMITTER: Agrawal A 

PROVIDER: S-EPMC7202941 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature