Δ⁹ -Tetrahydrocannabinolic acid alleviates collagen-induced arthritis: Role of PPARγ and CB₁ receptors.

ABSTRACT:

Background and purpose

Δ⁹ -Tetrahydrocannabinolic acid (Δ⁹ -THCA-A), the precursor of Δ⁹ -THC, is a non-psychotropic phytocannabinoid that shows PPARγ agonist activity. Here, we investigated the ability of Δ⁹ -THCA-A to modulate the classic cannabinoid CB₁ and CB₂ receptors and evaluated its anti-arthritis activity in vitro and in vivo.

Experimental approach

Cannabinoid receptors binding and intrinsic activity, as well as their downstream signalling, were analysed in vitro and in silico. The anti-arthritis properties of Δ⁹ -THCA-A were studied in human chondrocytes and in the murine model of collagen-induced arthritis (CIA). Plasma disease biomarkers were identified by LC-MS/MS based on proteomic an

SUBMITTER: Palomares B 

PROVIDER: S-EPMC7429492 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature