18F-FHBG PET-CT Reporter Gene Imaging of Adoptive CIK Cell Transfer Immunotherapy for Breast Cancer in a Mouse Model.
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ABSTRACT: Background
To further improve the efficiency of adoptively transferred cytokine-induced killer (CIK) cell immunotherapy in breast cancer (BC), a reliable imaging method is required to visualize and monitor these transferred cells in vivo.Methods
Herpes simplex virus 1-thymidine kinase (HSV1-TK) and 9-(4-[18F]fluoro-3-(hydroxymethyl)butyl)guanine (18F-FHBG) were used as a pair of reporter gene/reporter probe for positron emission tomography (PET) imaging in this study. Following the establishment of subcutaneous BC xenograft-bearing nude mice models, induced human CIK cells expressing reporter gene HSV1-TK through lentiviral transduction were intravenously injected to nude mice. γ-radioimmunoassay was used to determine the specific uptake of 18F-FHBG by these genetically engineered CIK cells expressing HSV1-TK in vitro, and 18F-FHBG micro positron emission tomography-computed tomography (PET-CT) imaging was performed to visualize these adoptively transferred CIK cells in tumor-bearing nude mice.Results
Specific uptake of 18F-FHBG by CIK cells expressing HSV1-TK was clearly observed in vitro. Consistently, the localization of adoptively transferred CIK cells in tumor target could be effectively visualized by 18F-FHBG micro PET-CT reporter gene imaging.Conclusion
PET-CT reporter gene imaging using 18F-FHBG as a reporter probe enables the visualization and monitoring of adoptively transferred CIK cells in vivo.
SUBMITTER: Li X
PROVIDER: S-EPMC7671474 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Publications
<sup>18</sup>F-FHBG PET-CT Reporter Gene Imaging of Adoptive CIK Cell Transfer Immunotherapy for Breast Cancer in a Mouse Model.
Li Xiaofeng X Yin Guotao G Ji Wei W Liu Jianjing J Zhang Yufan Y Wang Jian J Zhu Xiang X Zhu Lei L Dai Dong D Ma Wenchao W Xu Wengui W
OncoTargets and therapy 20201113
<h4>Background</h4>To further improve the efficiency of adoptively transferred cytokine-induced killer (CIK) cell immunotherapy in breast cancer (BC), a reliable imaging method is required to visualize and monitor these transferred cells in vivo.<h4>Methods</h4>Herpes simplex virus 1-thymidine kinase (<i>HSV1-TK</i>) and 9-(4-[<sup>18</sup>F]fluoro-3-(hydroxymethyl)butyl)guanine (<sup>18</sup>F-FHBG) were used as a pair of reporter gene/reporter probe for positron emission tomography (PET) imagin ...[more]
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