The IL-27/IL-27R axis is altered in CD4⁺ and CD8⁺ T lymphocytes from multiple sclerosis patients.

ABSTRACT:

Objectives

Pro- and anti-inflammatory properties have been attributed to interleukin-27 (IL-27). Nevertheless, the impact of this cytokine on chronic inflammatory diseases such as multiple sclerosis (MS) remains ill-defined. We investigated the biology of IL-27 and its specific receptor IL-27Rα in MS patients.

Methods

Levels of IL-27 and its natural antagonist (IL-27-Rα) were measured by ELISA in biological fluids. CD4⁺ and CD8⁺ T lymphocytes were isolated from untreated relapsing-remitting MS patients and healthy donors. Transcriptome-wide analysis compared T-cell subsets stimulated or not with IL-27. Expression of the IL-27Rα, key immune factors, STAT phosphorylation and cytokine production was assessed by flow cytometry.

Results

We observed el

SUBMITTER: Clenet ML 

PROVIDER: S-EPMC7934284 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature