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Clinical evaluation of the antibody response in patients with COVID-19 using automated high-throughput immunoassays.

ABSTRACT: Several automated high-throughput immunoassays for detecting anti-SARS-CoV-2 antibodies by a semi-quantitative approach have been commercialized. In this study, we describe the timeline of the antibody response in patients with RT-PCR-confirmed COVID-19. A total of 292 sequential serum samples from 33 Japanese patients were retrospectively analyzed using four test kits for SARS-CoV-2: the Abbott SARS-CoV-2 IgG assay (Abbott), Elecsys® Anti-SARS-CoV-2 assay (Roche Diagnostic), and VITROS® Anti-SARS-CoV-2 Total and IgG assays (Ortho Clinical Diagnostics). All automated immunoassays could equivalently identify positive sera collected within 2 weeks after symptom onset (99.3%-100%). In addition, the S protein-based automated immunoassay, the VITROS® Anti-SARS-CoV-2 Total assay, may play a complementary role in evaluating passive antibody therapies or vaccines against SARS-CoV-2, although further research is required.

SUBMITTER: Kubota K 

PROVIDER: S-EPMC7954771 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Clinical evaluation of the antibody response in patients with COVID-19 using automated high-throughput immunoassays.

Kubota Katsumi K   Kitagawa Yutaro Y   Matsuoka Masaru M   Imai Kazuo K   Orihara Yuta Y   Kawamura Rieko R   Sakai Jun J   Ishibashi Noriomi N   Tarumoto Norihito N   Takeuchi Shinichi S   Maesaki Shigefumi S   Maeda Takuya T  

Diagnostic microbiology and infectious disease 20210313 3

Several automated high-throughput immunoassays for detecting anti-SARS-CoV-2 antibodies by a semi-quantitative approach have been commercialized. In this study, we describe the timeline of the antibody response in patients with RT-PCR-confirmed COVID-19. A total of 292 sequential serum samples from 33 Japanese patients were retrospectively analyzed using four test kits for SARS-CoV-2: the Abbott SARS-CoV-2 IgG assay (Abbott), Elecsys® Anti-SARS-CoV-2 assay (Roche Diagnostic), and VITROS® Anti-SA  ...[more]

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