TSP-1 (Thrombospondin-1) Deficiency Protects ApoE^-/- Mice Against Leptin-Induced Atherosclerosis.

ABSTRACT:

Objective

Hyperleptinemia, hallmark of obesity, is a putative pathophysiologic trigger for atherosclerosis. We previously reported a stimulatory effect of leptin on TSP-1 (thrombospondin-1) expression, a proatherogenic matricellular protein implicated in atherogenesis. However, a causal role of TSP-1 in leptin-driven atherosclerosis remains unknown. Approach and Results: Seventeen-weeks-old ApoE^-/- and TSP-1^-/-/ApoE^-/- double knockout mice, on normocholesterolemic diet, were treated with or without murine recombinant leptin (5 µg/g bwt, IP) once daily for 3 weeks. Using aortic root morphometry and en face lesion assay, we found that TSP-1 deletion abrogated leptin-stimulated lipid-filled lesion burden, plaque area, and collagen accumulation in aorti

SUBMITTER: Ganguly R 

PROVIDER: S-EPMC8105272 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature