A FRET-based fluorescent Zn²⁺ sensor: 3D ratiometric imaging, flow cytometric tracking and cisplatin-induced Zn²⁺ fluctuation monitoring.

ABSTRACT: Monitoring labile Zn²⁺ homeostasis is of great importance for the study of physiological functions of Zn²⁺ in biological systems. Here we report a novel ratiometric fluorescent Zn²⁺ sensor, CPBT, which was constructed based on chelation-induced alteration of FRET efficiency. CPBT was readily cell membrane permeable and showed a slight preferential localization in the endoplasmic reticulum. With this sensor, 3D ratiometric Zn²⁺ imaging was first realized in the head of zebra fish larvae via Z-stack mode. CPBT could track labile Zn²⁺ in a large number of cells through ratiometric flow cytometric assay. More interestingly, both ratiometric fluorescence imaging and flow cytometric assay demonstrated that the labile Zn²⁺ l

SUBMITTER: Xu H 

PROVIDER: S-EPMC8162301 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature