Maturation conditions, post-ovulatory age, medium pH, and ER stress affect [Ca²⁺]i oscillation patterns in mouse oocytes.

ABSTRACT: Insufficiency of oocyte activation impairs the subsequent embryo development in assisted reproductive technology (ART). Intracellular Ca²⁺ concentration ([Ca²⁺]i) oscillations switch the oocytes to resume the second meiosis and initiate embryonic development. However, the [Ca²⁺]i oscillation patterns in oocytes are poorly characterized. In this study, we investigated the effects of various factors, such as the oocytes age, pH, cumulus cells, in vitro or in vivo maturation, and ER stress on [Ca²⁺]i oscillation patterns and pronuclear formation after parthenogenetic activation of mouse oocytes. Our results showed that the oocytes released to the oviduct at 17 h post-human chorionic gonadotrophin (hCG) displayed a significantly stronger [Ca^2+</

SUBMITTER: Yuan RY 

PROVIDER: S-EPMC8266968 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature