Mechanisms and consequences of casein kinase II and ankyrin-3 regulation of the epithelial Na⁺ channel.

ABSTRACT: Activity of the Epithelial Na⁺ Channel (ENaC) in the distal nephron fine-tunes renal sodium excretion. Appropriate sodium excretion is a key factor in the regulation of blood pressure. Consequently, abnormalities in ENaC function can cause hypertension. Casein Kinase II (CKII) phosphorylates ENaC. The CKII phosphorylation site in ENaC resides within a canonical "anchor" ankyrin binding motif. CKII-dependent phosphorylation of ENaC is necessary and sufficient to increase channel activity and is thought to influence channel trafficking in a manner that increases activity. We test here the hypothesis that phosphorylation of ENaC by CKII within an anchor motif is necessary for ankyrin-3 (Ank-3) regulation of the channel, which is required for normal channel locale and function, and

SUBMITTER: Abd El-Aziz TM 

PROVIDER: S-EPMC8285517 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature