M⁶ A modification of HSATIII lncRNAs regulates temperature-dependent splicing.

ABSTRACT: Nuclear stress bodies (nSBs) are nuclear membraneless organelles formed around stress-inducible HSATIII architectural long noncoding RNAs (lncRNAs). nSBs repress splicing of hundreds of introns during thermal stress recovery, which are partly regulated by CLK1 kinase phosphorylation of temperature-dependent Ser/Arg-rich splicing factors (SRSFs). Here, we report a distinct mechanism for this splicing repression through protein sequestration by nSBs. Comprehensive identification of RNA-binding proteins revealed HSATIII association with proteins related to N⁶ -methyladenosine (m⁶ A) RNA modification. 11% of the first adenosine in the repetitive HSATIII sequence were m⁶ A-modified. nSBs sequester the m⁶ A writer complex to methylate HSATIII, leading

SUBMITTER: Ninomiya K 

PROVIDER: S-EPMC8327944 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature