Establishment of a resource recycling strategy by optimizing isobutanol production in engineered cyanobacteria using high salinity stress.

ABSTRACT:

Background

Isobutanol is an attractive biofuel with many advantages. Third-generation biorefineries that convert CO₂ into bio-based fuels have drawn considerable attention due to their lower feedstock cost and more ecofriendly refining process. Although autotrophic cyanobacteria have been genetically modified for isobutanol biosynthesis, there is a lack of stable and convenient strategies to improve their production.

Results

In this study, we first engineered Synechococcus elongatus for isobutanol biosynthesis by introducing five exogenous enzymes, reaching a production titer of 0.126 g/L at day 20. It was then discovered that high salinity stress could result in a whopping fivefold increase in isobutanol production, with a maximal in-flask titer of 0.637 g/L at d

SUBMITTER: Wu XX 

PROVIDER: S-EPMC8404291 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature