An atlas of dynamic peripheral blood mononuclear cell landscapes in human perioperative anaesthesia/surgery.

ABSTRACT:

Background

The number of patients receiving anaesthesia is increasing, but the impact of general anaesthesia on the patient's immune system remains unclear. The aim of the present study is to investigate dynamics of systemic immune cell responses to anaesthesia during perioperative period at a single-cell solution.

Methods

The peripheral blood mononuclear cells (PBMCs) and clinical phenomes were harvested and recorded 1 day before anaesthesia and operation, just after anaesthesia (0 h), and 24 and 48 h after anaesthesia. Single-cell sequencing of PBMCs was performed with 10× genomics. Subsequently, data analysis was performed with R packages: Seurat, clusterProfiler and CellPhoneDB.

Results

We found that the cluster of CD56⁺ NK cells changed at 0 h and the

SUBMITTER: Wang YY 

PROVIDER: S-EPMC8782495 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature