Molecular and Genomic Insights of mcr-1-Producing Escherichia coli Isolates from Piglets.

ABSTRACT: The use of colistin in food-producing animals favors the emergence and spread of colistin-resistant strains. Here, we investigated the occurrence and molecular mechanisms of colistin resistance among E. coli isolates from a Mexican piglet farm. A collection of 175 cephalosporin-resistant colonies from swine fecal samples were recovered. The colistin resistance phenotype was identified by rapid polymyxin test and the mcr-type genes were screened by PCR. We assessed the colistin-resistant strains by antimicrobial susceptibility test, pulse-field gel electrophoresis, plasmid profile, and mating experiments. Whole-Genome Sequencing data was used to explore the resistome, virulome, and mobilome of colistin-resistant strains. A total of four colistin-resistant E. coli were i

SUBMITTER: Rodriguez-Santiago J 

PROVIDER: S-EPMC8868104 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature