Tertiary lymphoid structure and decreased CD8⁺ T cell infiltration in minimally invasive adenocarcinoma.

ABSTRACT: Knowledge of the tumor microenvironment (TME) in patients with early lung cancer, especially in comparison with the matched adjacent tissues, remains lacking. To characterize TME of early-stage lung adenocarcinoma, we performed RNA-seq profiling on 58 pairs of minimally invasive adenocarcinoma (MIA) tumors and matched adjacent normal tissues. MIA tumors exhibited an adaptive TME characterized by high CD4⁺ T cell infiltration, high B-cell activation, and low CD8⁺ T cell infiltration. The high expression of markers for B cells, activated CD4⁺ T cells, and follicular helper T (Tfh) cells in bulk MIA samples and three independent single-cell RNA-seq datasets implied tertiary lymphoid structures (TLS) formation. Multiplex immunohistochemistry staining validated

SUBMITTER: Wang J 

PROVIDER: S-EPMC8873609 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature