Measurement of circulating CD21^-CD27^- B lymphocytes in SLE patients is associated with disease activity independently of conventional serological biomarkers.

ABSTRACT: Determining disease activity in systemic lupus erythematosus (SLE) patients is challenging and limited by the lack of reliable biomarkers. Abnormally activated B cells play a key role in the pathogenesis of SLE, but their measure in clinical practice is currently not recommended. Here, we studied peripheral B cells to identify a valid biomarker. We analyzed peripheral B cells in a discovery cohort of 30 SLE patients compared to 30 healthy controls (HC) using mass cytometry and unsupervised clustering analysis. The relevant B cell populations were subsequently studied by flow cytometry in a validation cohort of 63 SLE patients, 28 autoimmune diseases controls and 39 HC. Our data show an increased frequency of B cell populations with activated phenotype in SLE compared to healthy and autoimm

SUBMITTER: Horisberger A 

PROVIDER: S-EPMC9163192 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature