Functional comparison of anti-restriction and anti-methylation activities of ArdA, KlcA, and KlcA_HS from Klebsiella pneumoniae.

ABSTRACT: Anti-restriction proteins are typically encoded by plasmids, conjugative transposons, or phages to improve their chances of entering a new bacterial host with a type I DNA restriction and modification (RM) system. The invading DNA is normally destroyed by the RM system. The anti-restriction proteins ArdA, KlcA, and their homologues are usually encoded on plasmid of carbapenemase-resistant Klebsiella pneumoniae. We found that the plasmid sequence and restriction proteins affected horizontal gene transfer, and confirmed the anti-restriction and anti-methylation activities of ArdA and KlcA during transformation and transduction. Among the three anti-restriction proteins, ArdA shows stronger anti-restriction and anti-methylation effects, and KlcA_HS was weaker. KlcA shows anti

SUBMITTER: Chen H 

PROVIDER: S-EPMC9366191 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature