Downregulation of IL-8 and IL-10 by the Activation of Ca²⁺-Activated K⁺ Channel K_Ca3.1 in THP-1-Derived M₂ Macrophages.

ABSTRACT: THP-1-differentiated macrophages are useful for investigating the physiological significance of tumor-associated macrophages (TAMs). In the tumor microenvironment (TME), TAMs with the M₂-like phenotype play a critical role in promoting cancer progression and metastasis by inhibiting the immune surveillance system. We examined the involvement of Ca²⁺-activated K⁺ channel K_Ca3.1 in TAMs in expressing pro-tumorigenic cytokines and angiogenic growth factors. In THP-1-derived M₂ macrophages, the expression levels of IL-8 and IL-10 were significantly decreased by treatment with the selective K_Ca3.1 activator, SKA-121, without changes in those of VEGF and TGF-β1. Furthermore, under in vitro experimental conditions that mimic extrac

SUBMITTER: Ohya S 

PROVIDER: S-EPMC9368915 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature