Development of a ⁶⁴Cu-labeled CD4⁺ T cell targeting PET tracer: evaluation of CD4 specificity and its potential use in collagen-induced arthritis.

ABSTRACT:

Background

CD4⁺ T cells are central inflammatory mediators in the pathogenesis of autoimmune rheumatoid arthritis (RA), as they are one of the dominating cell types in synovial inflammation. Molecular imaging of CD4⁺ T cells has potential role for early detection and monitoring of RA. Here, we developed a new radiotracer for in vivo immunoPET imaging of murine CD4⁺ T cells and tested it in the collagen-induced arthritis (CIA) mouse model of human RA.

Results

The tracer, [⁶⁴Cu]Cu-NOTA-CD4-F(ab)'2 ([⁶⁴Cu]Cu-NOTA-CD4), was generated from F(ab)'2 fragments of R-anti-mouse CD4 antibodies conjugated to the 2-S-(isothiocyanatbenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (p-SCN-Bn-NOTA) chelator and radiolabeled with copp

SUBMITTER: Clausen AS 

PROVIDER: S-EPMC9481863 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature