Dataset Information

Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.

ABSTRACT:

Objectives

Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear. We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort. We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation.

Design

We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.

Results

Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes. We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue. In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels. Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice. Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.

Conclusion

Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.

Trial registration number

NCT02059538.

SUBMITTER: Belda E

PROVIDER: S-EPMC9664128 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Publications

Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.

Belda Eugeni E Voland Lise L Tremaroli Valentina V Falony Gwen G Adriouch Solia S Assmann Karen E KE Prifti Edi E Aron-Wisnewsky Judith J Debédat Jean J Le Roy Tiphaine T Nielsen Trine T Amouyal Chloé C André Sébastien S Andreelli Fabrizio F Blüher Matthias M Chakaroun Rima R Chilloux Julien J Coelho Luis Pedro LP Dao Maria Carlota MC Das Promi P Fellahi Soraya S Forslund Sofia S Galleron Nathalie N Hansen Tue H TH Holmes Bridget B Ji Boyang B Krogh Pedersen Helle H Le Phuong P Le Chatelier Emmanuelle E Lewinter Christian C Mannerås-Holm Louise L Marquet Florian F Myridakis Antonis A Pelloux Veronique V Pons Nicolas N Quinquis Benoit B Rouault Christine C Roume Hugo H Salem Joe-Elie JE Sokolovska Nataliya N Søndertoft Nadja B NB Touch Sothea S Vieira-Silva Sara S Galan Pilar P Holst Jens J Gøtze Jens Peter JP Køber Lars L Vestergaard Henrik H Hansen Torben T Hercberg Serge S Oppert Jean-Michel JM Nielsen Jens J Letunic Ivica I Dumas Marc-Emmanuel ME Stumvoll Michael M Pedersen Oluf Borbye OB Bork Peer P Ehrlich Stanislav Dusko SD Zucker Jean-Daniel JD Bäckhed Fredrik F Raes Jeroen J Clément Karine K

Gut 20220111 12

<h4>Objectives</h4>Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear. We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort. We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metaboli ...[more]

PMID: 35017197

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Project description:Background and objectivesGut dysbiosis that resulted from the alteration between host-microbe interaction might worsen obesity-induced systemic inflammation. Gut microbiota manipulation by supplementation of prebiotic inulin may reverse metabolic abnormalities and improve obesity. This study aimed to determine whether inulin supplementation improved intestinal microbiota and microbial functional pathways in children with obesity.MethodsChildren with obesity whose BMI above median + 2SDs were recruited to a randomized, double-blinded placebo-controlled study. The participants aged 7-15 years were assigned to inulin supplement extracted from Thai Jerusalem artichoke (intervention), maltodextrin (placebo), and dietary fiber advice groups. All participants received similar monthly conventional advice and follow-up for 6 months. Fecal samples were collected for gut microbiome analysis using 16S rRNA sequencing. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States was performed to infer microbial functional pathways.ResultsOne hundred and forty-three children with available taxonomic and functional pathway abundance profiles were evaluated. A significant increase in alpha-diversity was observed in the inulin group. Inulin supplementation substantially enhanced Bifidobacterium, Blautia, Megasphaera, and several butyrate-producing bacteria, including Agathobacter, Eubacterium coprostanoligenes, and Subdoligranulum, compared to the other groups. The inulin group showed a significant difference in functional pathways of proteasome and riboflavin metabolism. These changes correlated with clinical and metabolic outcomes exclusively in the inulin group.ConclusionsInulin supplementation significantly promoted gut bacterial diversity and improved gut microbiota dysbiosis in children with obesity. The modulation of functional pathways by inulin suggests its potential to establish beneficial interactions between the gut microbiota and host physiology. Inulin supplementation could be a strategic treatment to restore the balance of intestinal microbiota and regulate their functions in childhood obesity.

Dataset Information

Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.

Objectives

Design

Results

Conclusion

Trial registration number

Publications

Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.

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