Chronic Ca²⁺ imaging of cortical neurons with long-term expression of GCaMP-X.

ABSTRACT: Dynamic Ca²⁺ signals reflect acute changes in membrane excitability, and also mediate signaling cascades in chronic processes. In both cases, chronic Ca²⁺ imaging is often desired, but challenged by the cytotoxicity intrinsic to calmodulin (CaM)-based GCaMP, a series of genetically-encoded Ca²⁺ indicators that have been widely applied. Here, we demonstrate the performance of GCaMP-X in chronic Ca²⁺ imaging of cortical neurons, where GCaMP-X by design is to eliminate the unwanted interactions between the conventional GCaMP and endogenous (apo)CaM-binding proteins. By expressing in adult mice at high levels over an extended time frame, GCaMP-X showed less damage and improved performance in two-photon imaging of sensory (whisker-deflection) response

SUBMITTER: Geng J 

PROVIDER: S-EPMC9699699 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature