Enrichment of hepatic glycogen and plasma glucose from H₂¹⁸ O informs gluconeogenic and indirect pathway fluxes in naturally feeding mice.

ABSTRACT: Deuterated water (² H₂ O) is a widely used tracer of carbohydrate biosynthesis in both preclinical and clinical settings, but the significant kinetic isotope effects (KIE) of ² H can distort metabolic information and mediate toxicity. ¹⁸ O-water (H₂ ¹⁸ O) has no significant KIE and is incorporated into specific carbohydrate oxygens via well-defined mechanisms, but to date it has not been evaluated in any animal model. Mice were given H₂ ¹⁸ O during overnight feeding and ¹⁸ O-enrichments of liver glycogen, triglyceride glycerol (TG), and blood glucose were quantified by ¹³ C NMR and mass spectrometry (MS). Enrichment of oxygens 5 and 6 relative to body water informed indirect pathwa

SUBMITTER: Coelho M 

PROVIDER: S-EPMC9845176 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature