A new and efficient enrichment method for metagenomic sequencing of Monkeypox virus.

ABSTRACT:

Background

The methodology described in previous literature for Monkeypox virus (MPXV) sequencing shows low efficiency when using metagenomic approaches. The aim of the present study was to evaluate a new fine-tuned method for extraction and enrichment of genomic MPXV DNA using clinical samples and to compare it to a non-enrichment metagenomic approach.

Results

A new procedure that allows sample enrichment in MPXV DNA, avoiding wasting the sequencing capacity in human DNA, was designed. This procedure consisted of host DNA depletion using a saponin/NaCl combination treatment and DNase, together with high g-force centrifugations. After typical quality control, samples using the enrichment method contained around 96% of reads not classified as human DNA, while the non-enrichme

SUBMITTER: Aja-Macaya P 

PROVIDER: S-EPMC9847149 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature