PET Imaging of Hepatocellular Carcinoma Using ZD2-(68Ga-NOTA).
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ABSTRACT: Purpose
We tested a recently developed short peptide radioligand for PET imaging of hepatocellular carcinoma (HCC) by targeting an oncoprotein, extra-domain B fibronectin (EDB-FN) in the tumor microenvironment.Methods
The radioligand consists of a small linear peptide ZD2 with 68Ga-NOTA chelator, and specifically binds to EDB-FN. PET images were acquired dynamically for 1 hour after intravenously (i.v.) injecting 37 MBq (1.0 mCi) of the radioligand into the woodchuck model of naturally occurring HCC. Woodchuck HCC originated from chronic viral hepatitis infection, which recapitulates the corresponding human primary liver cancer. The animals were euthanized post-imaging for tissue collection and validation.Results
For ZD2 avid liver tumors, the radioligand accumulation plateaued a few minutes after injection, while the liver background uptake stabilized 20 min post-injection. The status of EDB-FN in woodchuck HCC was confirmed by histology and validated by PCR and western blocking.Conclusion
We have showed the viability of using the ZD2 short peptide radioligand targeting EDB-FN in liver tumor tissue for PET imaging of HCC, which can potentially impact the clinical care for HCC patients.
SUBMITTER: Sergeeva O
PROVIDER: S-EPMC9968869 | biostudies-literature | 2023
REPOSITORIES: biostudies-literature
Publications
PET Imaging of Hepatocellular Carcinoma Using ZD2-(<sup>68</sup>Ga-NOTA).
Sergeeva Olga O Zhang Yifan Y Gao Songqi S Chan E Ricky ER Sergeev Maxim M Iyer Renuka R Sexton Sandra S Avril Norbert N Lu Zheng-Rong ZR Lee Zhenghong Z
Journal of hepatocellular carcinoma 20230222
<h4>Purpose</h4>We tested a recently developed short peptide radioligand for PET imaging of hepatocellular carcinoma (HCC) by targeting an oncoprotein, extra-domain B fibronectin (EDB-FN) in the tumor microenvironment.<h4>Methods</h4>The radioligand consists of a small linear peptide ZD2 with <sup>68</sup>Ga-NOTA chelator, and specifically binds to EDB-FN. PET images were acquired dynamically for 1 hour after intravenously (i.v.) injecting 37 MBq (1.0 mCi) of the radioligand into the woodchuck m ...[more]
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