The Hippo pathway drives the cellular response to hydrostatic pressure

ABSTRACT: Cells need to rapidly integrate multiple mechanical and chemical stimuli in order to ensure precise context dependent responses. This requires dynamic cellular signalling that ensure homeostasis and plasticity when needed. A less well understood process is cellular response to elevated interstitial fluid pressure. Here using quantitative label-free digital holographic imaging, combined with genome editing, biochemistry and confocal imaging, we analyse the temporal cellular response to cyclic hydrostatic pressure. Upon elevated cyclic hydrostatic pressure, the cell responds by rapid, dramatic and reversible changes in cellular volume. We show that YAP/TAZ, the co-transcriptional regulators of the Hippo signalling pathway, control cell volume and that cells without YAP/TAZ have lower plasma

SUBMITTER: Dr. Jiwon Park 

PROVIDER: S-SCDT-EMBOJ-2021-108719 | biostudies-other |

REPOSITORIES: biostudies-other