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NEUROD1 efficiently converts peripheral blood cells into neurons with partial reprogramming by pluripotency factors

ABSTRACT: The direct reprogramming of cells has tremendous potential in in vitro neurological studies. Previous attempts to convert blood cells into induced neurons have presented several challenges, necessitating the development of a less invasive, efficient, rapid, and convenient approach. The current study introduces an optimized method for converting somatic cells into neurons using a nonsurgical approach that employs peripheral blood cells instead of fibroblasts. We have demonstrated the efficacy of a unique combination of transcription factors, including NEUROD1, and four Yamanaka reprogramming factors (OCT3/4, SOX2, KLF4, and c-MYC), in generating glutamatergic neurons within a three-week period. This approach, which requires only five pivotal factors (NEUROD1, OCT3/4, SOX2, KLF4, and c-MYC), has the potential to create functional neurons and circumvents the need for induced pluripotent stem cell (iPSC) intermediates, as evidenced by gene expression and single-cell RNA sequencing (RNA-seq) analyses. Our method presents a rapid solution for modeling neuronal diseases with significant implications for drug discovery and personalized medicine and offers a patient-friendly alternative to traditional fibroblast reprogramming techniques.

ORGANISM(S): Homo sapiens

PROVIDER: GSE254389 | GEO | 2024/02/02

REPOSITORIES: GEO

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