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Disulfide Tethering to Map Small Molecule Binding Sites Transcriptome-Wide

ABSTRACT: Tether-seq uses s4U metabolic labeling to provide sites for reversible and covalent attachment of small molecule disulfides to the transcriptome. By screening under reducing conditions, we are able to highlight interactions that are stabilized by binding over those driven by the reactivity of the RNA sites. When applied to cellular RNA, Tether-seq with a disulfide analogue of risdiplam (compound 2) revealed a number of potential binding sites. Structure probing by SHAPE-MaP revealed a structured motif and confirmed binding to the lead molecule.

ORGANISM(S): Homo sapiens

PROVIDER: GSE261913 | GEO | 2025/05/01

REPOSITORIES: GEO

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Disulfide Tethering to Map Small Molecule Binding Sites Transcriptome-Wide

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