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TIMELESS promotes glioma stemness and malignancy through JAK-STAT3 pathway

ABSTRACT: RNA-sequencing (RNA-seq) analysis was performed to identify TIMELESS-regulated gene signatures in mouse glioblastoma (GBM) CT2A cells. Timeless was originally identified as a core component of the circadian clock machinery, but its role in GBM stemness and malignancy has not been defined. To investigate the mechanisms underlying Timeless-driven GBM malignancy, we generated CT2A cells with stable Timeless knockdown (KD) using a lentivirus expressing Timeless- specific shRNA, alongside CT2A cells transduced with a non-targeting scramble shRNA (shScramble) as a control. Total RNA was extracted from three independent biological replicates of shScramble and Timeless-KD CT2A cells, and bulk RNA barcoding and sequencing (BRB-seq) libraries were prepared and sequenced on the Illumina NextSeq2000 platform. Among the genes detected, genes whose mean expression levels in Timeless-KD cells were reduced to half or less of those in shScramble cells were selected and subjected to enrichment analysis using ShinyGO, which revealed significant enrichment of stem cell- related gene signatures. These findings suggest that TIMELESS regulates cancer stem-like properties of GBM cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE336084 | GEO | 2026/09/01

REPOSITORIES: GEO

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