Transcriptomics,Genomics

Dataset Information

37

Transcriptome of CD4+ T cell subsets


ABSTRACT: We compared the transcriptome of ex vivo-isolated murine naive CD4+ T cells, Th1 cells and Th17 cells by microarray. Overall design: CD4+ T effector cells were prepared ex vivo, stimulated with PMA/Ionomycin, subjected to a comercially available cytokine secretion kit (IL-17A and IFNg), stained by adding fluorescence-labeled antibodies against CD3, CD4 and CD45RB and sorted by flow cytometry. We sorted naive CD4+ T cells (CD3+CD4+CD45RB_high), Th1 cells (CD3+CD4+CD45RB_low_IFNg+IL17A-) and Th17 cells (CD3+CD4+CD45RB_low_IFNg-IL17A+).

INSTRUMENT(S): Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)

SUBMITTER: Robert Geffers  

PROVIDER: GSE58152 | GEO | 2014-12-31

SECONDARY ACCESSION(S): PRJNA251397

REPOSITORIES: GEO

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Publications

Development of a unique epigenetic signature during in vivo Th17 differentiation.

Yang Bi-Huei BH   Floess Stefan S   Hagemann Stefanie S   Deyneko Igor V IV   Groebe Lothar L   Pezoldt Joern J   Sparwasser Tim T   Lochner Matthias M   Huehn Jochen J  

Nucleic acids research 20150115 3


Activated naive CD4(+) T cells are highly plastic cells that can differentiate into various T helper (Th) cell fates characterized by the expression of effector cytokines like IFN-γ (Th1), IL-4 (Th2) or IL-17A (Th17). Although previous studies have demonstrated that epigenetic mechanisms including DNA demethylation can stabilize effector cytokine expression, a comprehensive analysis of the changes in the DNA methylation pattern during differentiation of naive T cells into Th cell subsets is lack  ...[more]

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