Metformin alters DNA methylation genome-wide via the H19/SAHH axis

ABSTRACT: To determine whether exposure of endometrial cancer cells to metformin would induce changes in global DNA methylation, ARK2 cells were incubated with metformin for 48 h, followed by genome-scale DNA methylation profiling using platform of an improved version of Reduced Representation Bisulfite Sequencing (RRBS). Metformin treatment led to extensive methylation changes genome-wide compared to control-treated cells. We found 2000 genes became hypermethylated, and 2000 genes became hypomethylated in ARK2 treatment group.

ORGANISM(S): Homo sapiens

PROVIDER: GSE85974 | GEO | 2016/09/01

SECONDARY ACCESSION(S): PRJNA339864

REPOSITORIES: GEO