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Omics score: 1005

Reproducible Automated Phosphopeptide Enrichment using Magnetic TiO2 and Ti-IMAC.

ABSTRACT: Data from ProteomeXchange, PXD ID: PXD000892. File: FIG5_1_KRAS_G12D_3.msf.mgf. Published as part of Anal Chem. 2014 Sep 18 . From the Abstract: {{i}} Reproducible, comprehensive phosphopeptide enrichment is essential to study phosphorylation-regulated processes. Here, we describe the application of hyper-porous magnetic TiO2 and Ti-IMAC microspheres for uniform automated phosphopeptide enrichment. Combining magnetic microspheres with a magnetic particle-handling robot enables rapid ..., reproducible ... and high-fidelity ... phosphopeptide purification in a 96-well format ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Mus_musculus_viruses, Mouse

DISEASE(S): Not Available

SUBMITTER: Tape CJ, et al.

PROVIDER: GPM32320003560 | GPMDB |

REPOSITORIES: GPMDB

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Reproducible automated phosphopeptide enrichment using magnetic TiO2 and Ti-IMAC.

Tape Christopher J CJ Worboys Jonathan D JD Sinclair John J Gourlay Robert R Vogt Janis J McMahon Kelly M KM Trost Matthias M Lauffenburger Douglas A DA Lamont Douglas J DJ Jørgensen Claus C

Analytical chemistry 20141002 20

Reproducible, comprehensive phosphopeptide enrichment is essential for studying phosphorylation-regulated processes. Here, we describe the application of hyper-porous magnetic TiO2 and Ti-IMAC microspheres for uniform automated phosphopeptide enrichment. Combining magnetic microspheres with a magnetic particle-handling robot enables rapid (45 min), reproducible (r2 ≥ 0.80) and high-fidelity (>90% purity) phosphopeptide purification in a 96-well format. Automated phosphopeptide enrichment demonst ...[more]

PMID: 25233145

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