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The S/T/Y-linked residue pairs identified by current software are unreliable

ABSTRACT: Here, by setting amino acids with chemically inert side chains such as glycine (G), valine (V), and leucine (L) as cross-linkable sites, which serves as a negative control, we show that the identifications of S/T/Y-linked peptides are as reliable as those of G/V/L-linked peptides. This is true across different NHS ester cross-linkers including DSS, DSSO, and DSBU, and across different search engines including MeroX, xiSearch, and pLink.

ORGANISM(S): Bos Taurus

SUBMITTER: Mengqiu Dong

PROVIDER: PXD039103 | iProX | Mon Dec 26 00:00:00 GMT 2022

REPOSITORIES: iProX

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