Proteomics

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The resistance mechanism of baohuoside I in Staphylococcus aureus


ABSTRACT: S. aureus isolate of baohuoside I resistance and YUSA145 was inoculated and cultured to exponential growth phase (OD600 at 0.5) in TSB washed twice with Phosphate Buffer Saline (PBS). Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer.

ORGANISM(S): Staphylococcus Aureus

SUBMITTER: Zewen Wen  

PROVIDER: PXD044989 | iProX | Thu Aug 31 00:00:00 BST 2023

REPOSITORIES: iProX

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