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Proteomic Analysis of a saline-alkali tolerant green microalga under NaCl stress

ABSTRACT: This project carried out a quantitative study of a saline-alkali tolerant green microalga under NaCl stress using iTRAQ labling and LC–MS/MS proteomic analysis. in order to ensurea high degre of credibility of the results, we quantitatively analyzed the changes. The standard for up-or down-regulated modification sites was set as a fold change>1.5 or <0.7, and p< 0.05, respectively. In this study, we carried out a systematic bioinformatics analysis of proteins containing quantitative information, including protein annotation, functional classification, functional enrichmentand cluster analysis based on functional enrichment.

ORGANISM(S): Monoraphidium Dybowskii

SUBMITTER: Chunxiang Hu

PROVIDER: PXD045064 | iProX | Sat Sep 02 00:00:00 BST 2023

REPOSITORIES: iProX

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Strategies of NaCl Tolerance in Saline-Alkali-Tolerant Green Microalga <i>Monoraphidium dybowskii</i> LB50.

Yang Haijian H Zhang Jing J Li Hua H

Plants (Basel, Switzerland) 20231007 19

Studying how freshwater cells modify metabolism and membrane lipids in response to salt stress is important for understanding how freshwater organisms adapt to salt stress and investigating new osmoregulatory ways. Physiological, biochemical, metabolic, and proteomic analyses were applied in a novel saline-alkali-tolerant microalga <i>Monoraphidium dybowskii</i> LB50 under different NaCl concentrations. Cells adopt a variety of strategies to adapt to salt stress, including increasing ion transpo ...[more]

PMID: 37836235

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