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Proteomics analysis for liver from WT and Hsd17b6 liver specific knockout mice

ABSTRACT: Eight weeks old LSL-Cas9 mice were injected with AAV2/8 expressing Cre driving by TBG promoter and sgRNA of gene of interest to specifically knockout gene in liver. In the present study, either control or sgHsd17b6 virus are injected. The mice are subjected to high fat choline dificient diet feeding for 8 weeks and then the liver samples were collected and subjected to Astral-DIA based proteomics analysis.

ORGANISM(S): Mus Musculus

SUBMITTER: Anyuan He

PROVIDER: PXD056581 | iProX | Wed Oct 02 00:00:00 BST 2024

REPOSITORIES: iProX

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Hepatic HSD17B6 is dispensable for diet-induced fatty liver disease in mice.

Yuan Delong D Bai Nan N Zhu Qihan Q Song Shaoxuan S He Anyuan A Wang Jianqing J Chen Yali Y

Biochemistry and biophysics reports 20250119

Metabolic dysfunction-associated fatty liver disease (MAFLD) affects up to a third of the global population, which causes huge both clinical and economic burdens. However, its therapeutic strategy is still limited. Steroid dysregulation plays a pivotal role in the homeostasis of lipid metabolism. 17-beta-hydroxysteroid dehydrogenase type 6 (HSD17B6)-one member of 17β-HSDs, encoded by the gene <i>Hsd17b6</i>, catalyzes the synthesis of androsterone and estrone-steroid hormones. However, whether t ...[more]

PMID: 39896111

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