Proteomics

Dataset Information

Proteins that interact with PHAX and UAP56 from HeLa nuclear extracts


ABSTRACT: HeLa nuclear extracts and RNase A were incubated with GST-PHAX and FLAG-UAP56 in the presence of ATP. The mixture was subjected to immunoprecipitation. The bound proteins were eluted with FLAG peptide. The eluate was subjected to GST pull-down. The bound proteins were treated with HRV 3C protease. Supernatants were analysed by SDS-PAGE and mass spectrometry.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Ichiro Taniguchi 

PROVIDER: PXD053480 | JPOST Repository | Sat Jun 28 00:00:00 GMT+01:00 2025

REPOSITORIES: jPOST