Dataset Information

Quantitative Proteomic Profiling of Normoxic and Hypoxic Neuroblastoma Cells during Co-culture

ABSTRACT: This dataset contains TMT-based quantitative proteomic profiles of fluorescence-activated cell sorting–isolated normoxic and hypoxic neuroblastoma cells collected from normoxic–hypoxic co-culture experiments. Three independent biological replicates were analyzed per condition. Proteins were extracted, digested with Lys-C and trypsin, labeled with TMT reagents, pooled, and fractionated by basic reversed-phase chromatography using C18 StageTips. LC–MS/MS analysis was performed using an Orbitrap Fusion Lumos mass spectrometer. The dataset was generated to characterize proteomic alterations associated with intercellular interactions and the adaptive response of hypoxic neuroblastoma cells during co-culture.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hsueh-Fen Juan

PROVIDER: PXD081335 | JPOST Repository | Wed Jul 22 00:00:00 BST 2026

REPOSITORIES: jPOST

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20220519_1462_TMU_Aki_TMTPro16x_R_F1.raw	Raw
	20220519_1463_TMU_Aki_TMTPro16x_R_F2.raw	Raw
	20220519_1464_TMU_Aki_TMTPro16x_R_F3.raw	Raw
	20220519_1465_TMU_Aki_TMTPro16x_R_F4.raw	Raw
	20220519_1467_TMU_Aki_TMTPro16x_R_F5.raw	Raw

Items per page:

1 - 5 of 17

Similar Datasets

Project description:Neuroblastoma is a common type of pediatric cancer, accounting for approximately 7% of childhood cancers. Hypoxia tumor environment caused by fast growing cancer cell, hypoxic cancer cell would cause cell death and cell proliferation rate down. Despite hypoxia causing tumor cell death and a decrease in cell proliferation rates, the hypoxic environment simultaneously activates various pathways, leading to the transformation of surviving hypoxic cells into malignant, proliferative tumors. Furthermore, these surviving cells may develop resistance to chemotherapy and radiotherapy.Tunneling nanotubes (TNTs) are intercellular conduits composed of F-actin that facilitate material transfer between cells.To understand the material transfer between normoxic and hypoxic cells via TNTs in the hypoxic tumor microenvironment, we conducted a TMT-based proteomic analysis of hypoxic and normoxic cells. TMT-Based proteomic analysis was used to investigate the changes in protein levels regulated via TNTs-mediated intercellular transfer between hypoxic and normoxic cells. We collected the conditional medium (CM) from the labeled normoxic (called normoxic CM) and hypoxic cells (called hypoxic CM), respectively. The hypoxic cells incubated with normoxic CM or normoxic cells incubated with hypoxic CM were used to exclude the influence of EVs and the soluble factors in the conditional medium. Following the FACS, six conditions of cells are all sorted and distinguished into two experimental batches, the normoxic to hypoxic batch (H batch: N-alone, H-alone, H (N CM), H-CO) and the hypoxic to normoxic batch (N batch: N-alone, N (H CM), N-CO). For TMT labeling, digested peptides from three biological replicates of the H batch and the N batch were labeled by TMTpro 16-plex kit and TMT 11-plex™ kit, respectively. The samples, sequentially analyzed by LC-MS/MS, were a total of 140159 MS/MS spectra, 46550 peptides, and 5835 proteins identified, and then we removed unreliable (potential contaminants) and low-quality (count ≤ 1) proteins. Finally, we got 4015 quantifiable proteins.

Dataset Information

Quantitative Proteomic Profiling of Normoxic and Hypoxic Neuroblastoma Cells during Co-culture

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets