Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Pos_acute_chagas_carnitine_ddms2

ABSTRACT: Positive polarity ddMS2 experiment of acute phase chagas diseases in mice treated with carnitine.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Mouse

SUBMITTER: Laura-Isobel McCall

PROVIDER: MSV000084869 | MassIVE | Thu Jan 30 09:59:00 GMT 2020

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

neg_acute_chagas_carnitine_ddms2

Project description:Negaive polarity ddMS2 experiment of Chagas disease acute phase in mice treated by carnitine

2020-01-30 | MSV000084870 | MassIVE

Massive_Swab_recovery_PRM_Pos_09262019

Project description:ddMS2 in positive polarity for swab recovery of chemicals from desk top after sampling different concentrations using PRM in Q Exactive Plus.

| MSV000084385 | MassIVE

Influenza_Lung_Plasma_Pos_ddMS2_05082020

Project description:ddMS2 run of mouse lung tissue and plasma extract using C8 column in 7.5-minute gradient and positive polarity mode in Q Exactive plus.

2020-05-08 | MSV000085389 | MassIVE

Ribosome profiling and RNA-seq of an acute glucose starvation timecourse and 5 day growth course in S. cerevisiae

Project description:Ribosome profiling provides an opportunity to not only assess how the relative abundance of ribosome association with mRNAs changes in different conditions, but to look more closely at where along mRNAs those ribosomes bind. Here, we used ribosome profiling to calculate the ribosome polarity scores and changes in ribosome footprint read density in both aggregate and gene-specific ways. We profiled a time course of acute glucose starvation followed by glucose readdition and a multi-day growth course through the diauxic shift into stationary phase. We found that ribosome polarity became positive in postdiauxic shift conditions relative to log phase. In acute starvation, polarity shifted positive at our earliest time points but did not continue to do so at later time points. This is consistent with a read density analysis which demonstrated increased density on the 3’ half of genes after glucose starvation. Additionally, we performed ribosome profiling in samples that had glucose added back following acute starvation and observed a wave of new ribosome movement near the start codon and approximately 2,000 nucleotides downstream on open reading frames after one and five minutes of readdition, respectively. Our ribosome profiling analysis suggested that elongation slows during starvation which leads to a buildup of ribosomes on the 3’ halves of mRNAs. Further, it also indicated ribosomes previously built up can resume translation upon glucose readdition. We used reporter assays to corroborate these findings in vivo. Together, these results demonstrate how yeast regulate translation in response to glucose starvation.

2022-04-11 | GSE200491 | GEO

GImap_negative_C8_7.5mint_chaoyi

Project description:apply C8 column and 7.5 minutes run to feces, small intestine and large intestine samples from mice under negative polarity mode in ddMS2.

2019-04-03 | MSV000083661 | MassIVE

Transcription profiling of Sachharomyces cerevisiae cultures following L-carnitine supplementation

Project description:Microarray analysis of the effect of L-carnitine supplementation on global expression of Sachharomyces cerevisiae cultures in logarithmic growing conditions and after exposure to H2O2 induced oxidative stress; L-Carnitine plays a well documented role in eukaryotic energy homeostasis by acting as a shuttling molecule for activated acyl residues across intracellular membranes. This activity is supported by carnitine acyl-transferases and transporters, and is referred to as the carnitine shuttle. However, several pleiotropic and often beneficial effects of carnitine in humans have been reported that appear to be unrelated to the shuttling activity, but little conclusive evidence regarding the molecular networks that would be affected by carnitine exist. We have recently demonstrated a protective role of carnitine in oxidative stress in yeast that is independent of the carnitine shuttle. A DNA microarray-based global gene expression analysis identified Cyc3p, a cytochrome c heme lyase, as being important for carnitine's protective impact in oxidative stress conditions. These findings establish a direct genetic link to a carnitine-related phenotype that is independent of the shuttle system. The data suggest that the yeast Saccharomyces cerevisiae should provide a useful model for further elucidation of carnitine's physiological roles. Experiment Overall Design: Yeast cultures was grown to mid-log phase with and without carnitine supplementation to a final concentration of 100 mg/L. A second set was grown to mid log phase, with and without carnitine supplementation and exposed to 0.4 mM H2O2 for 30 min. The experiments were performed using biological duplicate.

2009-07-04 | E-GEOD-16346 | biostudies-arrayexpress

C2C12_Treatment_with_Carnitine_ddMS2_QExactivePlus_Positive-Polarity_Polar_C18

Project description:ddMS2 data of C2C12 treated with Carnitine, untreated C2C12, water and carnitine in water using polar C18 column in Q Exactive Plus

2018-11-06 | MSV000083111 | MassIVE

GNPS_GImap_pos_C8_7.5mint_04032019_chaoyi

Project description:apply C8 column, 7.5 run to feces, small intestine and large intestine samples from mice under positive polarity mode in ddMS2.

2019-04-03 | MSV000083659 | GNPS

GNPS-acute stage Chagas disease carnitine treatment, large intestine (positive mode)

Project description:Large intestine samples from mice infected with 50,000 Trypanosoma cruzi parasites or left uninfected. One week post-infection, mice were treated with carnitine, benznidazole or vehicle. Animals were euthanized after 10 days of treatment and organs collected. Metabolites were extracted with 50% methanol followed by 3:1 dichloromethane-methanol and analyzed by C8 chromatography, with positive mode ddMS2 data collection (data-dependent).

2020-03-24 | MSV000085142 | GNPS

Neural stem and progenitor cells shorten S-phase upon commitment to neuron production

Project description:The dorsal telencephalon from E14.5 Tis21–GFP heterozygous mice embryos was subjected to microarray analysis. To identify genes whose expression were changed in Tis21–GFP positive S phase cells compared to Tis21–GFP negative S phase cells, Tis21–GFP positive or negative S phase cells labeled with a vital dye were separated by FACS corresponding to the GFP expression and DNA contents of the cells. 30 telencephalic hemispheres from 15 embryos of the Tis21–GFP heterozygous mice at E14.5 were dissected to collect approximately 200,000 S phase cells either from Tis21–GFP positive or negative for one replicate. Four independent experiments were performed from 3 different pregnant mice at least for each experiment set. Finally three best experiment sets were used for data analyses.

2010-11-30 | E-GEOD-23585 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data