The immunopeptidome of HLA-A*11:01 expressed K562 cell line

ABSTRACT: The K562 cell line was obtained from ATCC (American Type Culture Collection, Manassas,VA). And the K562 cell line was transduced using a highly efficient retroviral vector coding HLA-A*11:01. The vectors were transfected into a 293T packaging cell line and replication-defective virus supernatants were harvested. After infection of K562 cells with the supernatant, antibody-directed flow cytometry sorting was done to obtain high expression for HLA-A*11:01. Cells were grown in T75 flasks to a density of 1e9 cells before purification of HLA-I peptides for MS experiments.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Bo Li   Le Zhang  

PROVIDER: MSV000086567 | MassIVE | Mon Dec 07 02:56:00 GMT 2020

SECONDARY ACCESSION(S): PXD022951

REPOSITORIES: MassIVE