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Terminalia chebula decoction-processed Aconitum kusnezoffii preserves therapeutic effects through regulation of metabolism and intestinal microbiota

ABSTRACT:

Background: Aconitum kusnezoffii Reichb. is a commonly used medicinal herb in Mongolian medicine, a branch of traditional Chinese medicine. Processing A. kusnezoffii with a decoction of Terminalia chebula Retz. represents a characteristic and widely applied detoxification method in Mongolian medicine practice. Previous studies have demonstrated that this processing technique can reduce the toxicity of A. kusnezoffii. However, the underlying mechanisms remain insufficiently understood.

Methods: Normal Sprague-Dawley (SD) rats were randomly divided into the control (Ctrl), raw A. kusnezoffii (SCW), and Terminalia chebula decoction-processed A. kusnezoffii (HZZCW) groups. The metabolic profiles of rat feces and intestinal microbiota were detected. Hematoxylin and eosin (H&E) staining and enzyme-linked immunosorbent assay (ELISA) were performed to verify the toxicity of A. kusnezoffii. Western blotting (WB) and RT-qPCR were used to assess the expression of the PI3K/AKT signaling pathway. In addition, a collagen-induced arthritis (CIA) model was established to evaluate the anti-inflammatory effect.

Results: Metabolomic analysis revealed that processing A. kusnezoffii with Terminalia chebula decoction significantly altered metabolic pathways, particularly those related to ubiquinone biosynthesis, porphyrin metabolism, and pyrimidine metabolism. The results of 16S rDNA sequencing showed that the processed product markedly changed the alpha-diversity of the gut microbiota and reduced the relative abundance of Xanthomonas and Corynebacterium. Pharmacodynamic evaluation indicated that HZZCW reduced the toxicity of raw A. kusnezoffii by regulating the levels of malondialdehyde (MDA), lactate dehydrogenase (LDH), and superoxide dismutase (SOD), while activating the PI3K/AKT pathway to maintain its therapeutic efficacy. Furthermore, the CIA model confirmed its significant anti-inflammatory activity.

Conclusion: Terminalia chebula decoction-processed A. kusnezoffii may exert its pharmacodynamic effects by modulating ubiquinone biosynthesis, porphyrin metabolism, and pyrimidine metabolism, regulating intestinal microbiota, and activating the PI3K/AKT signaling pathway.

INSTRUMENT(S): Liquid Chromatography MS - alternating - reverse-phase

PROVIDER: MTBLS14867 | MetaboLights | 2026-06-27

REPOSITORIES: MetaboLights

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			Action	DRS
	a_MTBLS14867_LC-MS_alternating_reverse-phase.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS14867_LC-MS_alternating_reverse-phase_v2_maf.tsv	Tabular
	s_MTBLS14867.txt	Txt

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Terminalia chebula decoction-processed Aconitum kusnezoffii preserves therapeutic effects through regulation of metabolism and intestinal microbiota

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