Project description:C. albicans reference strain SC5314 was exposed to inhibitory concentration of ketoconzole. Seven mutants which obtained tolerance to ketoconazole were karyotyped by whole genome sequencing.
Project description:This SuperSeries is composed of the following subset Series: GSE24073: Transcriptional profile of Candida albicans during Hypoxic conditions. GSE24074: Transcriptional profile of Candida albicans DAY286 culture without ketoconazole versus DAY286 culture with 0.04 μg/ml ketoconazole, both at 20% oxygen (normoxia). GSE24075: Transcriptional profile of Candida albicans DAY286 versus UPC2 delete, both at 1% oxygen (hypoxia). Refer to individual Series
Project description:Candida albicans lab strain SC5314 was spread on YPD plates supplemented with ketoconazole (0.125-16μg/ml); Randomly 3 adaptors from each plate were sequenced.
Project description:We obtained ketoconazole tolerant mutants in Candida albicans after exposing the strain SC5314 to inhibitory concentration of ketoconazole. We did RNA-Seq to compare the transcriptomes of the aneuploid strains versus the parent euploid strain.
Project description:Candida albicans lab strain SC5314 was daily passaged in YPD broth supplemented with fluconazole. Some fluconazole-resistant and some fluconazole-tolerant adaptors were sequenced.
Project description:Candida albicans lab strain SC5314 was spread on YPD plates supplemented with echinocandins (caspofungin, anidulafungin, micafungin, rezafungin). The adaptors were tested for tolrance to fluconazole. Randomly 43 tolerant adaptors, as well as the parent SC5314, were sequenced.
Project description:Whole genome microarray were used to generate the transcriptional profile of C.albicans in the presence of ketoconazole. RNA was isolated from DAY286 grown with or without ketoconazole in YPD medium in normoxia at 30 degree Celsius for 3.5 h, and labeled with Cy3 or Cy5. Four independent biological replicates were compared. 2 dye swaps were perfomed so that 2 out of 4 samples isolated from DAY286 in normoxic culture without ketoconazole were labeled with Cy3, and 2 were labeled with Cy5.