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Nano HPLC Chip Ion Trap MS/MS of human pancreatic cancer cells

ABSTRACT: Proteins extracted from Panc1 cells (untreated or treated with ACPA or GW), were analyzed by 2-DE. Modulated proteins were identified by nanoHPLC Chip Ion trap. Bioinformatics pipeline: Mascot search was applied using the MS/MS ion search of Mascot against human entries of the non-redundant NCBI database, with propionamide formation of cysteines as fixed modification and oxidized methionine, acetylated protein N terminus, and phosphorylation of serine, threonine, and tyrosine as variable modifications. Trypsin was specified as the proteolytic enzyme and one missed cleavage was allowed. The mass tolerance of the precursor and fragment ions was set to +- 0.9 Da.

INSTRUMENT(S): instrument model

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Daniela Cecconi

PROVIDER: PXD000119 | Pride | 2014-07-10

REPOSITORIES: Pride

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Comparative proteomic and phosphoproteomic profiling of pancreatic adenocarcinoma cells treated with CB1 or CB2 agonists.

Brandi Jessica J Dando Ilaria I Palmieri Marta M Donadelli Massimo M Cecconi Daniela D

Electrophoresis 20130411 9-10

The pancreatic adenocarcinoma cell line Panc1 was treated with cannabinoid receptor ligands (arachidonylcyclopropylamide or GW405833) in order to elucidate the molecular mechanism of their anticancer effect. A proteomic approach was used to analyze the protein and phosphoprotein profiles. Western blot and functional data mining were also employed in order to validate results, classify proteins, and explore their potential relationships. We demonstrated that the two cannabinoids act through a wid ...[more]

PMID: 23463621

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