Proteomics

Dataset Information

38

Proteome analysis of Arabidopsis thaliana circadian clock mutants


ABSTRACT: The circadian clock is comprised of proteins that form negative feedback loops, which regulate the timing of global gene expression in a coordinated 24 hour cycle. As a result, the plant circadian clock is responsible for regulating numerous physiological processes central to growth and survival. To date, most plant circadian clock studies have relied on diurnal transcriptome changes to elucidate molecular connections between the circadian clock and observable phenotypes in wild-type plants. Here, we have combined high-throughput RNA-sequencing and mass spectrometry to comparatively characterize the lhycca1, prr7prr9, gi and toc1 circadian clock mutant rosette transcriptome and proteome at the end-of-day and end-of-night.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis thaliana  

TISSUE(S): Rosette

DISEASE(S): Not Available

SUBMITTER: Alexander Graf  

LAB HEAD: Alexander Graf

PROVIDER: PXD004763 | Pride | 2018-01-02

REPOSITORIES: Pride

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Publications

Parallel analysis of Arabidopsis circadian clock mutants reveals different scales of transcriptome and proteome regulation.

Graf Alexander A   Coman Diana D   Uhrig R Glen RG   Walsh Sean S   Flis Anna A   Stitt Mark M   Gruissem Wilhelm W  

Open biology 20170301 3


The circadian clock regulates physiological processes central to growth and survival. To date, most plant circadian clock studies have relied on diurnal transcriptome changes to elucidate molecular connections between the circadian clock and observable phenotypes in wild-type plants. Here, we have integrated RNA-sequencing and protein mass spectrometry data to comparatively analyse the lhycca1, prr7prr9, gi and toc1 circadian clock mutant rosette at the end of day and end of night. Each mutant a  ...[more]

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